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Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
Role of tryptophan in the pathogenesis of hepatic encephalopathy
Darakhshan M Saleem1, Saida Haider, Moona M Khan
1Department of Biomedical Engineering, Sir Syed University of Engineering Technology, Karachi.
Objective:
To monitor serum levels of total and free tyryptophan in various hepatic dysregulations.
Method:
The study was conducted on 36 adult patients. All were suffering from acute or chronic liver diseases such as hepatitis (n = 12), cirrhosis (n = 12) and Hepatic Encephalopathy (HE) (n = 12). Patients of all age groups and both genders were included. Serum levels of Tyryptophan (TRP) were measured by HPLC-EC. Albumin and bilirubin were analyzed by kit method (Merck).
Results:
The serum levels of total TRP (p < 0.01) was found in all patients. Free TRP significantly (p < 0.01) increased only in HE. Patients with hepatitis and cirrhosis did not show significant change in serum free TRP. The Albumin levels significantly decreased in hepatitis, cirrhosis and in HE (p < 0.01). All patients exhibited hypoalbuminaemia (p < 0.01).
Conclusions:
The results suggest that an increased ratio of free to bound TRP enhances its availability to the brain, which in turn increases 5-HT synthesis thus precipitating encephalopathy.
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