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Gas Chromatography: Types of Columns and Stationary Phases

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Related Experiment Video

Updated: Jul 6, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

Pharmaceutical applications on columns packed with sub-2 microm particles.

Rossana Russo¹, Davy Guillarme, Dao T-T Nguyen

¹Laboratory of Phytoanalysis, Dipartimento di Scienza e Tecnologia del Farmaco, Turin University, Via P. Giuria 9-10125 Turin, Italy.

Journal of Chromatographic Science

|March 13, 2008

Summary

Ultra-fast liquid chromatography using sub-2-micrometer particles offers enhanced separation power and faster analysis. These stable columns provide significant reductions in analysis time for pharmaceutical applications.

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Last Updated: Jul 6, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Published on: December 4, 2021

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

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Published on: August 28, 2015

Area of Science:

Analytical Chemistry
Chromatography

Background:

Growing interest in fast liquid chromatography (FLC).
Sub-2-micrometer particle size is a key strategy for improving separation power and analysis speed.

Purpose of the Study:

To evaluate the chromatographic performance and stability of sub-2-micrometer particle columns.
To demonstrate the potential for ultra-fast or highly efficient separations in pharmaceutical applications.

Main Methods:

Utilized plate-height curves to assess chromatographic performance.
Conducted system suitability experiments to evaluate column stability.
Tested columns under pressure conditions ranging from 200 to 800 bar.
Applied columns in both isocratic and gradient modes for pharmaceutical analyses.

Main Results:

Columns demonstrated stable performance over 1700 injections, maintaining retention, efficiency, and pressure.
Achieved 5- to 10-fold reduction in analysis time in isocratic mode with minimal impact on efficiency and resolution.
Reduced run times by up to 30-fold using shorter columns (30 mm).
Improved separation of pharmaceutical compounds and impurities in gradient mode using 100 mm columns, enhancing peak capacity and resolution.

Conclusions:

Sub-2-micrometer particle columns are stable and effective for fast and ultra-fast liquid chromatography.
These columns offer significant advantages in reducing analysis time and improving separation efficiency for pharmaceutical applications.