Related Experiment Video
Updated: Jul 6, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
Characteristics of superficially-porous silica particles for fast HPLC: some performance comparisons with
J J Destefano1, T J Langlois, J J Kirkland
1Advanced Materials Technology, Inc., 3521 Silverside Rd., Suite 1-K, Quillen Bldg., Wilmington, DE 19810, USA. joedestefano@advanced-materials-tech.com
Abstract:
Columns of 2.7-microm fused-core (superficially porous) Type B silica particles allow very fast separations of small molecules at pressures available in most high-performance liquid chromatography instruments. These highly-purified particles with 1.7-microm solid silica cores and 0.5-microm-thick shells of 9 nm pores exhibit efficiencies that rival those of totally porous sub-2-microm particles but at one-half to one-third of the column back pressure. This presentation describes other operating features of fused-core particle columns, including sample loading characteristics and packed bed stability. The superior mass transfer (kinetic) properties of the fused-core particles result in much-improved separation efficiency at higher mobile phase velocities, especially for > 600 molecular weight solutes.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Types of Detectors
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Elution Process
