Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compound cytotoxicity profiling using quantitative high-throughput screening.

Menghang Xia1, Ruili Huang, Kristine L Witt

  • 1NIH Chemical Genomics Center, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-3370, USA.

Environmental Health Perspectives
|March 13, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using sentiment analysis to quantify the relative desirability and acceptability of drug-product attributes.

JAMIA open·2026
Same author

KDM4C inhibition blocks tumor growth in basal breast cancer by promoting cathepsin L-mediated histone H3 cleavage.

Nature genetics·2025
Same author

Identifying Molecular Properties of Ataxin-2 Inhibitors for Spinocerebellar Ataxia Type 2 Utilizing High-Throughput Screening and Machine Learning.

Biology·2025
Same author

ZBTB7A is a modulator of KDM5-driven transcriptional networks in basal breast cancer.

Cell reports·2024
Same author

Small-Molecule Disruptors of the Interaction between Calcium- and Integrin-Binding Protein 1 and Integrin α<sub>IIb</sub>β<sub>3</sub> as Novel Antiplatelet Agents.

ACS pharmacology & translational science·2024
Same author

A call from 40 public health scientists for an end to the continuing humanitarian and environmental catastrophe in Gaza.

Environmental health : a global access science source·2024
Same journal

A New Start.

Environmental health perspectives·2026
Same journal

Time-Varying Exposure to Element Mixtures and Children's Cognition at 5 Years of Age: Findings from the New Hampshire Birth Cohort Study.

Environmental health perspectives·2026
Same journal

Effect of Household Air Pollution on the Gut Microbiome and Virome of Adult Women Living in Uganda.

Environmental health perspectives·2026
Same journal

Comparison of Temperature-Mortality Associations across the Middle East Using Different Exposure Estimation Approaches.

Environmental health perspectives·2026
Same journal

Workflow for Statistical Analysis of Environmental Mixtures.

Environmental health perspectives·2026
Same journal

Effects of Extreme Heat Exposure on Heatstroke and Liver Injury in Mice: The Role of PPARα.

Environmental health perspectives·2026
See all related articles

This study uses quantitative high-throughput screening (qHTS) to assess compound cytotoxicity in human and rodent cells. The cell-based assays offer a promising alternative to animal testing for prioritizing chemical safety evaluations.

Area of Science:

  • Toxicology
  • Cell Biology
  • High-Throughput Screening

Background:

  • Traditional animal-based toxicity testing is expensive, low-throughput, and raises ethical concerns.
  • Species differences in biology can limit the accuracy of extrapolating animal toxicity data to human health effects.

Purpose of the Study:

  • To identify a battery of cell-based screens for prioritizing compounds in toxicologic evaluations.
  • To develop and validate quantitative high-throughput screening (qHTS) methods for cytotoxicity assessment.

Main Methods:

  • 1,408 compounds were profiled for cytotoxicity using qHTS across 13 human and rodent cell types.
  • Cell types were derived from six major organs targeted by xenobiotics: liver, blood, kidney, nerve, lung, and skin.
  • Cytotoxic compounds were further analyzed for response kinetics.
Keywords:
1,536-wellNTP 1,408 compound libraryPubChemRT-CEScell viabilityqHTS

Related Experiment Videos

Main Results:

  • qHTS generated robust and reproducible cytotoxicity data, enabling cross-compound, cross-cell type, and cross-species comparisons.
  • Observed cytotoxicity patterns varied, with some compounds affecting all cell types while others showed species- or cell type-specific effects.
  • Kinetic studies revealed distinct time-dependent responses for compounds with similar cytotoxicity levels, correlating with known toxicity mechanisms.

Conclusions:

  • High-quality cytotoxicity data generated via qHTS on a large compound library shows the potential of this methodology.
  • Cell-based screening can prioritize compounds for further toxicologic evaluation and identify mechanisms of action.
  • This approach may aid in predicting in vivo biological responses, offering a more efficient and ethical alternative to traditional methods.