Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 6, 2026

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

Efficient stem cell labeling for MRI studies.

E Küstermann1, U Himmelreich, K Kandal

  • 1Max-Planck-Insitute for Neurological Research with Klaus-Joachim-Zülch-Laboratories of the Max-Planck-Society and the Faculty of Medicine of the University of Cologne, Germany.

Contrast Media & Molecular Imaging
|March 13, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome.

Acta neuropathologica communications·2024
Same author

Targeted next-generation sequencing of circulating free DNA enables non-invasive tumor detection in myxoid liposarcomas.

Molecular cancer·2022
Same author

Oral health knowledge and practices in the Kaski District of Nepal.

Community dental health·2021
Same author

Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.

Archives of orthopaedic and trauma surgery·2020
Same author

Motor cortex metabolite alterations in amyotrophic lateral sclerosis assessed in vivo using edited and non-edited magnetic resonance spectroscopy.

Brain research·2019
Same author

Non-invasive assessment of disease progression and neuroprotective effects of dietary coconut oil supplementation in the ALS SOD1<sup>G93A</sup> mouse model: A <sup>1</sup>H-magnetic resonance spectroscopic study.

NeuroImage. Clinical·2018
Same journal

RETRACTION: Clinical Value Study on Contrast-Enhanced Ultrasound Combined with Enhanced CT in Early Diagnosis of Primary Hepatic Carcinoma.

Contrast media & molecular imaging·2026
Same journal

Correction to "Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients".

Contrast media & molecular imaging·2026
Same journal

RETRACTION: Structural and Functional Characterization at the Molecular Level of the MATE Gene Family in Wheat in Silico.

Contrast media & molecular imaging·2025
Same journal

RETRACTION: The Significance of PAX8-PPARγ Expression in Thyroid Cancer and the Application of a PAX8-PPARγ-Targeted Ultrasound Contrast Agent in the Early Diagnosis of Thyroid Cancer.

Contrast media & molecular imaging·2025
Same journal

RETRACTION: COVID-19 Semantic Pneumonia Segmentation and Classification Using Artificial Intelligence.

Contrast media & molecular imaging·2025
Same journal

RETRACTION: Intelligent Algorithm-Based Ultrasound Images in Evaluation of Therapeutic Effects of Radiofrequency Ablation for Liver Tumor and Analysis on Risk Factors of Postoperative Infection.

Contrast media & molecular imaging·2025
See all related articles

This study compared iron oxide particles for labeling stem cells, finding that label efficiency and stability depend heavily on the specific particles and cell types used. Long-term effects on cell viability were also assessed.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Iron oxide nanoparticles offer high molar relaxivity, making them suitable for cell tracking.
  • Various iron oxide formulations exist, each with potentially different labeling characteristics.

Purpose of the Study:

  • To compare the suitability of different iron oxide particles (Sinerem, Endorem, magnetic microspheres) for labeling embryonic stem cells (D3 cell line).
  • To evaluate the long-term stability (up to 4 weeks) and potential toxicity of iron oxide labeling on cell viability.
  • To assess label efficiency and stability across different cell types, including neural progenitor cells (C17.2) and dendritic cells.

Main Methods:

  • Labeling of embryonic stem cells (D3), neural progenitor cells (C17.2), and dendritic cells with Sinerem, Endorem, and magnetic microspheres.

More Related Videos

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
11:41

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Published on: August 3, 2008

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

Related Experiment Videos

Last Updated: Jul 6, 2026

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
11:41

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Published on: August 3, 2008

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

  • Assessment of detectability thresholds for each iron oxide particle type.
  • Evaluation of long-term labeling stability (up to 4 weeks) and cell viability assays to determine toxic effects.

    • Main Results:

    • Significant variations in labeling efficiency and stability were observed among the different iron oxide particles tested.
    • The effectiveness and longevity of cell labeling were found to be highly dependent on the specific iron oxide particle formulation and the cell line used.
    • No universal optimal iron oxide particle was identified; suitability is context-dependent.

    Conclusions:

    • The choice of iron oxide particles is critical for successful and stable cell labeling in stem cell tracking applications.
    • Cell type significantly influences the efficiency and long-term stability of iron oxide labeling.
    • Further research is needed to optimize iron oxide particle selection for specific cell tracking applications and minimize potential adverse effects.