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Nuclear factor I gene expression in the developing forebrain.

Céline Plachez1, Charlotta Lindwall, Nana Sunn

  • 1University of Maryland, Baltimore, School of Medicine, Baltimore, Maryland, USA.

The Journal of Comparative Neurology
|March 13, 2008
PubMed
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Nuclear Factor I (Nfi) genes, NFIA and NFIB, are crucial for mouse brain development. While essential for midline glial development, they also play cell-autonomous roles in corticospinal neuron development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Nuclear Factor I (Nfi) gene family members, including Nfia, Nfib, and Nfix, are highly expressed during mouse brain development.
  • Previous studies show Nfia and Nfib knockout mice have significant defects in midline glial populations and forebrain commissure development, suggesting a role in axon guidance.
  • Nfi genes are also expressed in the cerebral cortex, necessitating an investigation into potential cell-autonomous roles in cortical development.

Purpose of the Study:

  • To investigate the protein expression patterns of NFIA and NFIB during embryonic and postnatal mouse forebrain development.
  • To determine if NFIA and NFIB expression correlates with cell-autonomous functions in cortical development.
  • To clarify the roles of NFIA and NFIB in both the formation of axon guidance substrates and neuronal development.

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Main Methods:

  • Detailed analysis of NFIA and NFIB protein expression in the developing mouse forebrain using immunohistochemistry.
  • Examination of expression patterns during both embryonic and postnatal developmental stages.
  • Correlation of NFIA and NFIB expression with specific neuronal populations, including callosally and laterally projecting neurons.

Main Results:

  • NFIA and NFIB proteins are expressed in deep cortical layers and the subplate during prenatal development, with dynamic postnatal expression.
  • High expression of NFIA and NFIB is observed in the developing hippocampus and diencephalon.
  • While NFIA and NFIB are largely absent in callosally projecting neurons postnatally, they are expressed in a significant proportion of laterally projecting neurons.

Conclusions:

  • The expression patterns of NFIA and NFIB support their role in regulating axon guidance substrates via midline glial populations.
  • The expression of NFIA and NFIB in laterally projecting neurons suggests a cell-autonomous role in corticospinal neuron development.
  • Collectively, NFIA and NFIB are implicated in both guiding axonal growth and directly influencing cortical neuron development.