Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The German National Strategy for Gene- and Cell-Based Therapies: Generating Impact by Employing a Novel Multi-Stakeholder Approach.

Human gene therapy·2026
Same author

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same author

Capsid-engineered AAV vector overcomes a key intracellular barrier and efficiently transduces spiral ganglion neurons in adult mice.

Molecular therapy. Advances·2026
Same author

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same author

Therapeutic antibody delivery: vector tools to boost efficacy and affordability.

Frontiers in immunology·2026
Same author

Robust Functionality and Regulation of Selectively Expressed RNA as AAV Vectors and In Vitro Transcribed Molecules.

Pharmaceutics·2025

Related Experiment Video

Updated: Jul 6, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Artificial evolution with adeno-associated viral libraries.

Luca Perabo1, Anke Huber, Stephan Märsch

  • 1Clinic I, Internal Medicine, University of Cologne, D-50937 Cologne, Germany.

Combinatorial Chemistry & High Throughput Screening
|March 14, 2008
PubMed
Summary
This summary is machine-generated.

Adeno-associated virus (AAV) vectors are promising for gene therapy. Combinatorial engineering and high-throughput screening accelerate AAV vector development and investigate viral infection mechanisms.

More Related Videos

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
06:41

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

Published on: October 20, 2023

Related Experiment Videos

Last Updated: Jul 6, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
06:41

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

Published on: October 20, 2023

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • Adeno-associated virus (AAV) is a promising vector for human gene therapy due to its favorable characteristics.
  • Extensive research over two decades has led to standard technologies for AAV genome manipulation, packaging, and purification.
  • Limited understanding of virus-cell interaction mechanisms has hindered progress in AAV vector development.

Purpose of the Study:

  • To review the rationale and accomplishments of using combinatorial methods in AAV vector development.
  • To discuss the potential of these methods in overcoming current limitations.
  • To explore future developments in AAV vector technology.

Main Methods:

  • Review of existing literature on AAV vector development and combinatorial engineering.
  • Discussion of high-throughput selection techniques for screening AAV clones.
  • Analysis of reverse genetics approaches for studying viral infectious biology.

Main Results:

  • Combinatorial engineering and high-throughput selection offer powerful tools for AAV vector improvement.
  • These methods can accelerate technological advancement with less reliance on detailed viral biology knowledge.
  • They also serve as valuable tools for investigating the molecular mechanisms of viral infection.

Conclusions:

  • Combinatorial approaches are crucial for advancing AAV vector technology in gene therapy.
  • Further development and application of these methods will enhance our understanding of viral-host interactions.
  • Future research should focus on integrating these techniques to overcome current limitations and unlock new possibilities in gene transfer.