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Characterization of a candidate bcl-1 gene
D A Withers1, R C Harvey, J B Faust
1Department of Medicine, University of California, San Francisco.
Molecular and Cellular Biology
|October 1, 1991
Summary
Researchers identified the bcl-1 gene, a cyclin family member, linked to human B-lymphocytic malignancy. This gene
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- The t(11;14)(q13;q32) translocation is linked to human B-lymphocytic malignancy.
- This translocation joins the immunoglobulin heavy-chain gene to the bcl-1 locus.
- The bcl-1 gene, a potential dominant oncogene, remained unidentified.
Purpose of the Study:
- To identify and characterize the bcl-1 gene.
- To investigate the role of bcl-1 in cell cycle regulation and oncogenesis.
Main Methods:
- Isolation of genomic clones from the bcl-1 locus.
- Identification of a candidate bcl-1 gene using specific probes.
- Cloning of bcl-1 mRNA cDNAs from cell lines.
Main Results:
- The bcl-1 gene was identified and found to be a member of the cyclin gene family.
- bcl-1 mRNA is widely expressed in human cell lines, except in lymphoid or myeloid lineages.
- The deduced amino acid sequence confirmed bcl-1's cyclin family membership.
Conclusions:
- The bcl-1 gene is a cyclin, potentially acting as a dominant oncogene.
- Altered cell cycle control due to bcl-1 activation may drive oncogenesis.
- Differential bcl-1 expression suggests distinct cell cycle regulation mechanisms in various tissues.