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Related Concept Videos

The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Microtubule Associated Motor Proteins01:32

Microtubule Associated Motor Proteins

Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...
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Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
Microtubules in Cell Motility01:24

Microtubules in Cell Motility

Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Microtubules in Cell Motility

Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Toxic effects of zinc ions on kinesin - Potential molecular cause of impaired intracellular transport.

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Related Experiment Video

Updated: Jul 6, 2026

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
10:46

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Published on: February 2, 2022

Kinesin-driven transport in cell-free environment.

Konrad J Böhm1

  • 1Leibniz Institute for Age Research, Fritz Lipmann Institute, Molecular Motors Lab, Beutenbergstrasse 11, D-07745 Jena, Germany. kboehm@fli-leibniz.de

Cell Biology International
|March 14, 2008
PubMed
Summary

Conventional kinesin (motor protein) transports cellular cargo along microtubules. Neuron-specific kinesin KIF5A also promotes microtubule assembly, even without pure tubulin.

Area of Science:

  • Cell Biology
  • Molecular Motors
  • Cytoskeletal Dynamics

Background:

  • Conventional kinesin is a key ATPase-active motor protein.
  • It mediates microtubule-dependent transport of cytoplasmic cargoes within cells.
  • Understanding kinesin's function is crucial for cell biology.

Purpose of the Study:

  • To provide an overview of kinesin-1 and its motility generation.
  • To investigate the role of neuron-specific kinesin KIF5A in microtubule formation.
  • To explore kinesin's function outside of a living cell context.

Main Methods:

  • Literature review and overview of kinesin-1 motility.
  • Experimental investigation of neuron-specific kinesin KIF5A.
  • Analysis of microtubule assembly under varying conditions.

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Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
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Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

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Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Related Experiment Videos

Last Updated: Jul 6, 2026

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
10:46

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Published on: February 2, 2022

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Main Results:

  • Kinesin-1's motility generation outside a living cell is summarized.
  • Neuron-specific kinesin KIF5A demonstrates the ability to enable microtubule formation.
  • Microtubule assembly was observed even when pure tubulin was absent.

Conclusions:

  • Kinesin-1 plays a significant role in cellular transport.
  • Neuron-specific kinesin KIF5A possesses microtubule-nucleating properties.
  • Kinesin proteins have diverse functions beyond cargo transport, including cytoskeletal regulation.