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Related Experiment Videos

Multiple human D5 dopamine receptor genes: a functional receptor and two pseudogenes.

D K Grandy1, Y A Zhang, C Bouvier

  • 1Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University.

Proceedings of the National Academy of Sciences of the United States of America
|October 15, 1991
PubMed
Summary

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Researchers discovered the functional human dopamine D5 receptor, closely related to the D1 receptor. Two other identified genes are non-functional pseudogenes, suggesting recent evolutionary events in dopamine receptor evolution.

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • The D1 dopamine receptor plays a crucial role in various brain functions.
  • Understanding the human dopamine receptor family is essential for neuroscience research.

Purpose of the Study:

  • To identify and characterize novel genes related to the D1 dopamine receptor in the human genome.
  • To investigate the functional and evolutionary relationships between these genes.

Main Methods:

  • Human genome sequencing and gene identification.
  • Functional expression studies in cell lines (COS-7 and 293 cells).
  • Nucleotide sequence analysis and comparison.
  • Blot hybridization on rat genomic DNA.

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Main Results:

  • Identified three human genes related to the D1 dopamine receptor.
  • One gene encodes a functional dopamine D5 receptor with higher dopamine affinity and distinct cAMP signaling compared to D1.
  • Two genes were identified as pseudogenes due to premature termination codons, showing high sequence identity to D5.
  • Evidence suggests a single D5 gene in rats and recent evolutionary origins for the pseudogenes.

Conclusions:

  • The human genome harbors a functional dopamine D5 receptor, distinct from D1.
  • The identified pseudogenes represent a recent evolutionary event in dopamine receptor gene family.
  • Further research into D5 receptor function may reveal new therapeutic targets.