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Decreased erythrocyte (Ca2+ + Mg2+)-ATPase activity in hemodialyzed uremic patients

O Shalev1

  • 1Department of Medicine, Hadassah University Hospital-Mount Scopus Jerusalem, Israel.

Renal Failure
|January 1, 1991
PubMed

Insights

Erythrocyte calcium pump activity is reduced in hemodialyzed patients, leading to increased intracellular calcium. This erythrocyte (Ca2+ + Mg2+)-ATPase malfunction may contribute to calcium buildup in uremia.

Area of Science:

  • Biochemistry
  • Nephrology
  • Cell Physiology

Background:

  • Uremia is associated with altered cellular ion transport.
  • Erythrocyte (Ca2+ + Mg2+)-ATPase plays a crucial role in maintaining calcium homeostasis.
  • Intracellular calcium accumulation is a known complication in uremic patients.

Purpose of the Study:

  • To investigate erythrocyte (Ca2+ + Mg2+)-ATPase activity and calcium content in hemodialyzed uremic patients.
  • To determine if erythrocyte calcium pump function is impaired in uremia.
  • To explore the potential link between erythrocyte calcium handling and systemic calcium dysregulation in uremia.

Main Methods:

  • Erythrocyte (Ca2+ + Mg2+)-ATPase activity was measured using biochemical assays.
  • Erythrocyte calcium content was determined using spectrophotometric methods.
  • Comparisons were made between 15 hemodialyzed uremic patients and 15 healthy controls.

Main Results:

  • Erythrocytes from hemodialyzed patients exhibited significantly lower (Ca2+ + Mg2+)-ATPase activity compared to controls (65 +/- 7 vs. 79 +/- 12 mumol Pi/g Hb/h, p < 0.001).
  • A corresponding significant increase in erythrocyte calcium content was observed in patients (17.2 +/- 6.4 vs. 5.1 +/- 4.2 mumol/L RBC, p < 0.05).

Conclusions:

  • Erythrocyte (Ca2+ + Mg2+)-ATPase activity is impaired in hemodialyzed uremic patients.
  • This malfunction contributes to the observed increase in intracellular calcium.
  • Systemic (Ca2+ + Mg2+)-ATPase dysfunction may underlie the pathophysiology of calcium accumulation in uremia.

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