[Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC]
Yan-Rong Li1, Ye Jiang, Fu Hao
1School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.
Objective:
To establish a RP-HPLC method for fast and simultaneous determination of four index contents, which are Genpioside, Baicalin, Berberine Hydrochloride and Ammonium Glyeyrrhizinate, in Qingwei Huanglian tablets.
Method:
A separation was well achieved by gradient elution on a Hypersil C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phases of acetontrile -0.5% triethylamine water-solution (pH 3.0) at a flow rate of 1.0 mL x min(-1), detection wavelength of 230 nm, and room temperature.
Result:
The calibration curve were linear in the ranges of 4.82-77.2, 5.80-92.8, 1.63-26.1 and 6.40-102 microg x mL(-1) (r = 0.9997-0.9999) for genpioside, baicalin, berberine hydrochloride and ammonium glyeyrrhizinate, respectively. The average recoveries of four index components were not less than 98.0%.
Conclusion:
The method is convenient, rapid and accurate. It is suitable for the quality control of Qingwei Huanglian tablets.
