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Updated: Jul 6, 2026

Custom-made Microdialysis Probe Design
Published on: July 21, 2015
Comparison of microdialysis with solid-phase microextraction for in vitro and in vivo studies
Simon Ningsun Zhou1, Gangfeng Ouyang, Janusz Pawliszyn
1Department of Chemistry, University of Waterloo, Waterloo, Ont, Canada.
Abstract:
Microdialysis (MD) and solid-phase microextraction (SPME) methods have been developed and compared through in vitro and in vivo studies. For in vitro study, both methods offered accurate and precise results for complex sample matrices by standard addition method. Compared to MD, the fully automated SPME procedure offered several advantages including high-throughput and more efficient sampling, less labor intensity, and capability for batch analysis. For in vivo study, although both techniques provided sampling with minimal perturbation to the system under study, SPME was more sensitive, precise and accurate, suitable for field sampling and had a wider application than MD. It demonstrated that SPME has the potential to replace MD for in vivo study.
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