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Related Experiment Video

Updated: Jul 6, 2026

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
11:29

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Published on: October 3, 2012

A sensitive and quantitative assay for normal PrP in plasma.

Luisa Gregori1, Benjamin N Gray, Elaine Rose

  • 1Veterans Affairs Medical Center and University of Maryland, Baltimore, MD 21201, United States. lgreg002@umaryland.edu

Journal of Virological Methods
|March 15, 2008
PubMed
Summary

A new blood screening assay can detect transmissible spongiform encephalopathies (TSEs) by identifying abnormal prion protein (PrP) in plasma. This sensitive assay offers a potential method to mitigate disease spread via blood transfusions.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Medical Diagnostics

Background:

  • Transmissible spongiform encephalopathies (TSEs) pose a risk of transmission through blood transfusions.
  • Developing a reliable blood screening assay is crucial for mitigating human disease spread.

Purpose of the Study:

  • To develop a sensitive assay for detecting prion protein (PrP) in plasma.
  • To improve the detection limit and sample volume for PrP detection.

Main Methods:

  • Utilized a two-antibody assay with ORIGEN technology and protocol modifications.
  • Increased sample volume to 1 mL for enhanced detection sensitivity.
  • Employed Western blot and immuno-affinity resin for plasma PrP(c) detection.

Main Results:

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Related Experiment Videos

Last Updated: Jul 6, 2026

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
11:29

Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain

Published on: October 3, 2012

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
07:13

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Published on: January 7, 2019

  • Achieved a detection limit of 3 pg in 1 mL plasma volume.
  • Measured PrP concentration in normal and infected hamster brains (7.5 ± 0.9 and 57.3 ± 9.6 µg/g).
  • Estimated abnormal PrP concentration in infected hamster plasma at 32 fg/mL.

Conclusions:

  • Developed a novel and sensitive assay for PrP detection in plasma.
  • The assay shows potential for a plasma-based TSE diagnostic test.
  • Mitigating TSE transmission risk through blood transfusion screening is feasible.