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Updated: Jul 6, 2026

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
Published on: January 24, 2025
Application of in-house mortality composting on viral inactivity of Newcastle disease virus
E R Benson1, G W Malone, R L Alphin
1Department of Bioresources Engineering, University of Delaware, Newark 19716, USA
Abstract:
This paper summarizes the results from 3 simulated in-house catastrophic mortality composting experiments. Experiment 1 evaluated the impact of water-based foam mass depopulation on in-house composting of the carcasses and litter and showed that water-based foam improved windrow temperatures. Experiment 2 evaluated the impact of freezing samples on virus recovery from windrow compost tissue and the choice of tissue for virus sampling within the bird. Experiment 2 documented that freezing the samples had minimal impact on processing and that virus recovery was more consistent among inoculated breast meat than inoculated tracheas. Experiment 3 evaluated the impact of sawdust, straw, and sawdust-straw base layer litter material on in-house mortality composting. All litter materials were able to reach and maintain temperatures in excess of 60 degrees C for multiple days. No viral hemagglutination activity was observed after d 2 during any of the 3 experiments.

