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Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

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Related Experiment Video

Updated: Jul 6, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Microplate-compatible biamperometry array for parallel 48-channel amperometric or coulometric measurements.

Thomas S Mann¹, Liam O'Hagan, Peter Ertl

¹Rapid Laboratory Microsystems Incorporated, Freeport Health Centre, P.O. Box 9056, 3570 King Street East, Kitchener, Ontario, Canada N2P 2G5.

Analytical Chemistry

|March 18, 2008

Summary

This summary is machine-generated.

A novel reusable electrochemical array enables rapid, parallel biamperometric measurements in standard microplates. This high-throughput system accurately quantifies various analytes and enzymes, offering a precise and cost-effective solution for biochemical assays.

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Area of Science:

Electrochemistry
Analytical Chemistry
Biotechnology

Background:

Standard electrochemical methods can be time-consuming and lack high-throughput capabilities.
Parallel analysis is crucial for increasing efficiency in biochemical assays and diagnostics.

Purpose of the Study:

To develop and validate a reusable electrochemical array for parallel biamperometric measurements.
To demonstrate the array's utility in quantifying various oxidizable species and in enzymatic assays.

Main Methods:

Design of a 48-channel electrochemical array using platinum wire electrodes in a standard 96-well microplate format.
Biamperometric detection employing potassium ferricyanide as an oxidant for various analytes.
Application to enzymatic assays, exemplified by glucose oxidase for glucose quantitation.

Main Results:

Accurate quantitation of acetaminophen, ascorbic acid, hydroquinone, trolox, and uric acid with detection limits down to 1 microM.
Linearity observed up to 100 microM for tested analytes.
Achieved a detection limit of 0.1 mM for glucose in enzymatic assays within 20 minutes.

Conclusions:

The developed electrochemical array offers a simple, precise, and rapid high-throughput method for biochemical analysis.
The array is suitable for a wide range of applications, including oxidase-based assays.
The use of standard microplates makes this a cost-effective and versatile tool.