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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Related Experiment Video

Updated: Jul 6, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Raman multiplexers for alternative gene splicing.

Lan Sun1, Chenxu Yu, Joseph Irudayaraj

  • 1Department of Agricultural and Biological Engineering and Bindley Bioscience Center, Purdue University, 225 South University Street, West Lafayette, Indiana 47907, USA.

Analytical Chemistry
|March 18, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for detecting breast cancer susceptibility gene 1 (BRCA1) alternative splice variants using surface-enhanced Raman scattering (SERS) with nonfluorescent labels. This highly sensitive technique offers a new tool for comprehensive gene splicing analysis.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

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Last Updated: Jul 6, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Alternative splicing of the breast cancer susceptibility gene 1 (BRCA1) contributes to cancer development.
  • Accurate detection of BRCA1 splice variants is crucial for understanding cancer pathogenesis.

Purpose of the Study:

  • To develop a novel, highly sensitive method for multiplex detection of BRCA1 alternative splice junctions.
  • To demonstrate the feasibility of using surface-enhanced Raman scattering (SERS) with nonfluorescent labels for this purpose.

Main Methods:

  • Utilized an array-format multiplex detection system.
  • Employed nonfluorescent labels for surface-enhanced Raman scattering (SERS) analysis.
  • Designed DNA probes specific to four distinct BRCA1 alternative splice variants: Delta(11q), Delta(9, 10), Delta(5), and Delta(5q, 6).

Main Results:

  • Achieved a four-plex detection of BRCA1 alternative splice variants.
  • Demonstrated high detection sensitivity of up to 1 femtomolar (fM).
  • Successfully discriminated splice junctions through specific target identification using Raman labels.

Conclusions:

  • This study presents the first proof-of-concept for SERS-based detection of alternative gene splicing using nonfluorescent labels.
  • The developed approach shows potential as a sensitive and selective tool for comprehensive alternative splicing profiling of BRCA1 and other disease-relevant genes.