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Related Experiment Videos

CD45RA antibodies split the CD3bright T cell subset.

S Ezine1, J Marvel, E Lightstone

  • 1INSERM U 25, Hôpital Necker, Paris, France.

International Immunology
|September 1, 1991
PubMed
Summary
This summary is machine-generated.

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CD45RA expression on thymocytes, including T cell precursors and mature cells, is further characterized in mice. This marker is present throughout development and is upregulated in cortisone-treated or irradiated mice.

Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Thymocyte subsets are traditionally defined by CD4 and CD8 expression.
  • Recent advances using anti-CD3 antibodies enable more precise phenotyping.
  • CD45RA expression on thymocytes suggests a role in defining progenitor populations and continuous lineages.

Purpose of the Study:

  • To further characterize the CD45RA-expressing thymocyte population in mice.
  • To investigate the role of CD45RA in thymocyte development and maturation.
  • To analyze CD45RA expression under different physiological and experimental conditions.

Main Methods:

  • Utilized a panel of CD45RA antibodies for characterization.
  • Employed three-colour immunofluorescence for detailed analysis.

Related Experiment Videos

  • Examined thymocytes from normal, cortisone-treated, and sublethally irradiated mice, as well as fetal and adult stages.

    • Main Results:

    • The CD45RA+ thymocyte subset is significantly expanded in cortisone-treated and irradiated mice.
    • CD45RA expression is observed early in fetal life and maintained throughout development.
    • In normal thymus, CD45RA+ cells are mainly in the CD4-CD8- subset; however, after cortisone treatment or irradiation, all four thymocyte subsets co-express CD45RA.
    • A substantial proportion of CD45RA+ cells are CD3bright and present across all tested maturation stages.
    • Both fetal TCR-gamma delta + CD3+ and adult TCR-alpha beta+ CD3+ cells can co-express CD45RA.

    Conclusions:

    • CD45RA is expressed on both progenitor and mature thymocyte populations, particularly those surviving stress conditions.
    • CD45RA expression is a consistent feature throughout thymocyte development.
    • The findings suggest CD45RA is a valuable marker for studying thymocyte lineage and maturation, including diverse T cell receptor expressing populations.