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Sequence analysis of the protein-coding regions of foot-and-mouth disease virus O/HK/2001

Zhao Mingqiu1, Suo Qingli, Chen Jinding

  • 1College of Veterinary Medicine, South China Agricultural University, Guangzhou 510640, China.

Veterinary Microbiology
|March 18, 2008
PubMed

Insights

Foot-and-mouth disease virus (FMDV) strain O/HK/2001, prevalent in Hong Kong, shows genetic links to Taiwan isolates. This molecular analysis aids in understanding FMDV type O regional variations.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Foot-and-mouth disease virus (FMDV) poses a significant threat to livestock globally.
  • Understanding the genetic diversity of FMDV strains is crucial for effective disease control.
  • Previous FMDV outbreaks in Hong Kong highlight the need for continuous molecular surveillance.

Purpose of the Study:

  • To determine the nucleotide sequence of the FMDV strain O/HK/2001.
  • To compare O/HK/2001 with other FMDV sequences for phylogenetic analysis.
  • To identify genetic variations and establish a database for molecular epidemiology.

Main Methods:

  • Nucleotide sequencing of the FMDV O/HK/2001 protein-coding region.
  • Bioinformatic analysis including sequence comparison and phylogenetic tree construction.
  • Identification of mutations, including substitutions and deletions, within the viral genome.

Main Results:

  • The protein-coding region of O/HK/2001 is 6966 nucleotides, encoding a 2322 amino acid protein.
  • O/HK/2001 belongs to the Cathay topotype and is closely related to FMDV isolates from Taiwan (1997) and HKN/2002.
  • Key mutations were identified, including substitutions in VP1 and L proteins, and a 10-amino acid deletion in the 3A protein.

Conclusions:

  • The FMDV strain O/HK/2001 causing outbreaks in Hong Kong is genetically linked to Taiwanese isolates.
  • The identified mutations may influence viral characteristics and transmission.
  • This sequence data is valuable for FMDV molecular epidemiological investigations and surveillance.

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