Related Experiment Video
Updated: Aug 6, 2026

Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols
Published on: April 4, 2014
Dihydroxyacetone reductase from Mucor javanicus. 1. Isolation and properties
Researchers purified an oxidoreductase enzyme from Mucor Javanicus fungus, identifying it as an e-Si oxidoreductase. This enzyme is stereospecific and useful for creating pure chiral alcohols and ketones.
Area of Science:
- Biochemistry
- Enzymology
- Mycology
Background:
- An NADPH-dependent oxidoreductase was extracted and purified from Mucor Javanicus.
- The enzyme's molecular weight and subunit composition were determined.
Purpose of the Study:
- To characterize the oxidoreductase from Mucor Javanicus.
- To determine the enzyme's stereospecificity and potential applications in chiral synthesis.
Main Methods:
- Enzyme purification via centrifugation and gel filtration.
- Electrophoresis for subunit analysis.
- Kinetic studies and product analysis using trans-1-decalones and trans-1-decalols.
- Stereospecificity determination using labeled coenzyme.
Main Results:
- The enzyme was purified 1000-fold and characterized.
- It was identified as an e-Si oxidoreductase, showing high stereospecificity.
- The enzyme demonstrated utility in preparing optically pure chiral alcohols and ketones.
Conclusions:
- The Mucor Javanicus oxidoreductase is a stereospecific enzyme with potential applications in asymmetric synthesis.
- Further studies suggest dihydroxyacetone as its natural substrate.
More Related Videos
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10:21Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Published on: June 20, 2019
Related Concept Videos
Alcohols from Carbonyl Compounds: Reduction
Catalytic hydrogenation is similar to the reduction of an alkene or alkyne by adding H2 across the pi bond in the presence of transition metal catalysts like Raney Ni, Pd–C, Pt, or Ru. Aldehydes and ketones can be reduced by this method, often under mild to moderate heat (25–100°C) and...
Preparation of Diols and Pinacol Rearrangement
The reaction begins with transferring a proton from the acid catalyst to one of the hydroxyl groups, producing an oxonium ion.
Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids
Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H] allows...
Carboxylic Acids to Primary Alcohols: Hydride Reduction
Protecting Groups for Aldehydes and Ketones: Introduction
α-Hydroxy Ketones via Reductive Coupling of Esters: Acyloin Condensation Overview