Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dihydroxyacetone reductase from Mucor javanicus. 1. Isolation and properties.

H Dutler, J L Van der Baan, E Hochuli

    European Journal of Biochemistry
    |May 16, 1977
    PubMed
    Summary

    Researchers purified an oxidoreductase enzyme from Mucor Javanicus fungus, identifying it as an e-Si oxidoreductase. This enzyme is stereospecific and useful for creating pure chiral alcohols and ketones.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pre-procedural image-guided versus non-image-guided ventricular tachycardia ablation-a review.

    Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·2020
    Same author

    Scintillator screen development for fast neutron radiography and tomography and its application at the beamline of the 10 MW BNC research reactor.

    Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2018
    Same author

    Fast neutron radiography and tomography at a 10MW research reactor beamline.

    Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2016
    Same author

    Genetic risk and longitudinal disease activity in systemic lupus erythematosus using targeted maximum likelihood estimation.

    Genes and immunity·2016
    Same author

    The contribution of the European high containment laboratories during the 2014-2015 Ebola Virus Disease emergency.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2016
    Same author

    Evidence for the founder effect of a novel ACVRL1 splice-site mutation in Hungarian hereditary hemorrhagic telangiectasia families.

    Clinical genetics·2016

    Area of Science:

    • Biochemistry
    • Enzymology
    • Mycology

    Background:

    • An NADPH-dependent oxidoreductase was extracted and purified from Mucor Javanicus.
    • The enzyme's molecular weight and subunit composition were determined.

    Purpose of the Study:

    • To characterize the oxidoreductase from Mucor Javanicus.
    • To determine the enzyme's stereospecificity and potential applications in chiral synthesis.

    Main Methods:

    • Enzyme purification via centrifugation and gel filtration.
    • Electrophoresis for subunit analysis.
    • Kinetic studies and product analysis using trans-1-decalones and trans-1-decalols.
    • Stereospecificity determination using labeled coenzyme.

    Main Results:

    Related Experiment Videos

    • The enzyme was purified 1000-fold and characterized.
    • It was identified as an e-Si oxidoreductase, showing high stereospecificity.
    • The enzyme demonstrated utility in preparing optically pure chiral alcohols and ketones.

    Conclusions:

    • The Mucor Javanicus oxidoreductase is a stereospecific enzyme with potential applications in asymmetric synthesis.
    • Further studies suggest dihydroxyacetone as its natural substrate.