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Induction of CD8 molecules on thymic gamma/delta T cells in vitro is dependent upon alpha/beta T cells

A L Spetz1, P Kourilsky, E L Larsson-Sciard

  • 1Biologie Moléculaire du Gène, U 277 INSERM, UA 535 CNRS, Stockholm, Stockholm.

European Journal of Immunology
|November 1, 1991
PubMed

Thymocytes differentiate upon interactions with microenvironmental components, but the precise role of different stromal cells, or other T cells, in early differentiative events remains unclear. Here we have analyzed the in vitro differentiation of double-negative (DN) thymocytes from young adult mice. We demonstrate that a substantial proportion of DN thymocytes differentiate into CD8+ gamma/delta T cells upon stimulation with concanavalin A and recombinant interleukin 2. However, if alpha/beta T cells are excluded from the initial population of DN thymocytes, the CD8+ gamma/delta T cells do not appear in the cultures. These results suggest a role for T-T cell interactions in thymic differentiative events, and provide evidence for physiological interactions between the alpha/beta and gamma/delta T cell compartments within the thymus.

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