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Updated: Jul 6, 2026

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 27, 2016
Genome-wide mapping of allele-specific protein-DNA interactions in human cells
Nathaniel D Maynard1, Jing Chen, Rhona K Stuart
1Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Ave., La Jolla, California 92037, USA.
Abstract:
We describe a high-throughput method, named ChIP-SNP, for the identification of allele-specific protein-DNA interactions throughout the human genome. ChIP-SNP combines chromatin immunoprecipitation (ChIP) with whole-genome single nucleotide polymorphism (SNP) genotyping microarray analysis. We demonstrated that it can be used to accurately identify allele-specific binding of RNA polymerase II (RNAP) in the human fibroblast genome, uncovering imprinted genes and other allele-specific binding events. ChIP-SNP will facilitate the study of mechanisms of allele-specific gene expression.
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