Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variation on Zernike's phase-contrast microscope.

R Liang, J K Erwin, M Mansuripur

    Applied Optics
    |March 18, 2008
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Current status and progress of staging and efficacy evaluation of marginal zone lymphoma].

    Zhonghua yi xue za zhi·2026
    Same author

    [A case report of prolonged pneumonia].

    Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024
    Same author

    [Treatment status of tyrosine kinase inhibitor for newly-diagnosed chronic myeloid leukemia: a domestic multi-centre retrospective real-world study].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2024
    Same author

    [Advances in the use of neuromuscular electrical stimulation in the prevention of venous thromboembolism].

    Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024
    Same author

    [Calculus around tracheotomy tube in a child: a case report].

    Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2024
    Same author

    [To compare the efficacy and incidence of severe hematological adverse events of flumatinib and imatinib in patients newly diagnosed with chronic phase chronic myeloid leukemia].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2023
    Same journal

    Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

    Applied optics·2026
    Same journal

    High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

    Applied optics·2026
    Same journal

    Automated stitching interferometry for high-precision metrology of X-ray mirrors.

    Applied optics·2026
    Same journal

    Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

    Applied optics·2026
    Same journal

    High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

    Applied optics·2026
    Same journal

    Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

    Applied optics·2026
    See all related articles

    This study presents a novel Zernike phase-contrast microscope. The innovative design allows for adjustable phase shifts and amplitude ratios, enabling quantitative phase measurements of samples.

    Area of Science:

    • Optical microscopy
    • Phase contrast imaging
    • Interferometry

    Background:

    • Traditional Zernike phase-contrast microscopy has limitations in controlling amplitude and phase.
    • Accurate quantitative phase measurements are crucial for various scientific applications.

    Purpose of the Study:

    • To design, construct, and test a new variant of the Zernike phase-contrast microscope.
    • To enable adjustable amplitude ratios and phase shifts between test and reference beams.
    • To facilitate quantitative phase measurements of sample surfaces.

    Main Methods:

    • Utilized a white-light source with an annular aperture.
    • Separated diffracted test beams from reference beams within the objective lens.
    • Combined beams after relative phase shifting to form an interferogram on a CCD camera.

    Related Experiment Videos

    Main Results:

    • Successfully constructed and tested a functional Zernike phase-contrast microscope variant.
    • Demonstrated adjustable amplitude ratio and phase shift capabilities.
    • Achieved quantitative phase measurement of the sample's imparted phase shift.

    Conclusions:

    • The developed microscope offers enhanced control over phase contrast imaging.
    • The adjustable parameters allow for versatile bright-field, dark-field, and phase-contrast microscopy.
    • This system provides a platform for precise quantitative phase analysis in microscopy.