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Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview

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Updated: Jul 6, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Published on: September 8, 2010

DNA double-strand breaks: a potential causative factor for mammalian aging?

Han Li1, James R Mitchell, Paul Hasty

  • 1Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, 15355 Lambda Drive, San Antonio, TX 78245-3207, USA. hli@cnio.es

Mechanisms of Ageing and Development
|March 19, 2008
PubMed
Summary

DNA double-strand breaks (DSBs) are toxic and may cause aging. Faulty DSB repair leads to age-dependent decline, suggesting different aging mechanisms in mouse models.

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Detection of DNA Double-Stranded Breaks in Mouse Oocytes
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Last Updated: Jul 6, 2026

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
13:10

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Published on: September 8, 2010

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

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Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Area of Science:

  • Gerontology
  • Molecular Biology
  • Genetics

Background:

  • Aging is a complex process influenced by genetics and environment.
  • Genome maintenance, especially DNA repair, is crucial for longevity.
  • DNA double-strand breaks (DSBs) are highly toxic DNA lesions.

Purpose of the Study:

  • To explore DNA DSBs as a potential cause of aging.
  • To discuss factors generating DSBs, repair pathways, and consequences of faulty repair.
  • To compare mouse models with defects in DSB or UV lesion repair.

Main Methods:

  • Review of literature on DNA damage and aging.
  • Analysis of factors contributing to DSB generation.
  • Examination of DNA repair pathways and their role in aging.
  • Comparative analysis of premature aging mouse models.

Main Results:

  • Imperfect genome maintenance, particularly DSB repair, may contribute to aging.
  • Faulty DSB repair can lead to age-dependent decline in fitness.
  • Mouse models with DSB repair defects show different aging phenotypes compared to those with UV lesion repair defects.

Conclusions:

  • DNA DSBs are a significant factor to consider in aging research.
  • The fundamental mechanisms underlying aging phenotypes can be distinct.
  • Aging is a pleiotropic process with diverse underlying causes.