Specific assay for unconjugated dehydroepiandrosterone in human plasma by capillary gas chromatography with
L Chabraoui1, B Mathian, M C Patricot
1Department of Hormonology, Centre Hospitalier Lyon-Sud, Pierre Benite, France.
Abstract:
A specific and sensitive method for the determination of unconjugated dehydroepiandrosterone in plasma is described. After extraction and purification of the extracts on a Celite column, the iodomethyldimethylsilyl ether derivative of dehydroepiandrosterone was isolated on an aluminium oxide column and assayed by gas chromatography with electron-capture detection. The method is sensitive: sample volumes of 0.5-1 ml are sufficient for the determination of dehydroepiandrosterone in plasma of normal male and female subjects aged 1-80 years. The assay is highly specific and has the potential to be used as a reference method for the determination of unconjugated dehydroepiandrosterone in biological samples.
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