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Pharmacokinetics in Pediatric Patients: Drug Excretion

Pharmacokinetics in Pediatric Patients: Drug Excretion

In pediatric medicine, understanding the renal function and drug elimination nuances is crucial for administering safe and effective treatments. Newborns, in particular, display markedly slower renal functions than adults, profoundly affecting how drugs are cleared from their bodies. This slower drug clearance requires clinicians to extend the dosing intervals for many medications to prevent drug accumulation and toxicity while ensuring therapeutic efficacy.One key area where these adjustments...

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Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

HPLC-fluorescence assay for acyclovir in children.

L Zeng¹, C E Nath, P J Shaw

¹Faculty of Pharmacy, University of Sydney, NSW, Australia.

Biomedical Chromatography : BMC

|March 19, 2008

Summary

This study presents a new HPLC method for accurately measuring acyclovir in human plasma. The validated assay is sensitive and reliable for quantifying acyclovir levels in pediatric patients.

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Area of Science:

Pharmacokinetics and Drug Monitoring
Analytical Chemistry
Clinical Diagnostics

Background:

Accurate quantification of antiviral drugs like acyclovir is crucial for effective patient treatment.
Existing methods for acyclovir measurement may lack the sensitivity or reliability required for pediatric populations.
Therapeutic drug monitoring of acyclovir ensures optimal dosage and minimizes toxicity.

Purpose of the Study:

To develop and validate a simple, accurate, reliable, and sensitive High-Performance Liquid Chromatography (HPLC) method for quantifying acyclovir in human plasma.
To establish the method's performance characteristics, including linearity, accuracy, precision, and limits of detection and quantitation.
To assess the applicability of the developed method for measuring acyclovir concentrations in pediatric oncology patients.

Main Methods:

A C18 HPLC column was utilized with a mobile phase consisting of sodium phosphate buffer and acetonitrile.
Fluorescence detection was employed with specific excitation and emission wavelengths.
Sample preparation involved protein precipitation using perchloric acid and the addition of guanosine as an internal standard.

Main Results:

The developed HPLC method demonstrated high analyte recovery (101%) and linearity over the concentration range of 0.1-20 mg/L.
Intra- and inter-day accuracy and precision were consistently below 7%.
The limit of detection was 0.033 mg/L, and the limit of quantitation was 0.1 mg/L, indicating high sensitivity.

Conclusions:

The validated HPLC method is suitable for the accurate and sensitive quantification of acyclovir in human plasma.
The method's performance characteristics make it ideal for therapeutic drug monitoring, particularly in pediatric patients.
This assay provides a valuable tool for managing acyclovir therapy in pediatric oncology patients, ensuring optimal drug exposure and safety.