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Induction of apoptosis by recombinant soluble human TRAIL in Jurkat cells

Gen-Hong Yao1, Li-Jun Ling, Jian-Feng Luan

  • 1Department of Transfusion, Jinling Hospital, Medical School, Nanjing University, Nanjing 210002, Jiangsu, China. ygh71@tom.com

Biomedical and Environmental Sciences : BES
|March 20, 2008
PubMed
Abstract

Insights

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) shows therapeutic potential by inhibiting cancer cell proliferation. TRAIL effectively induces apoptosis, a programmed cell death pathway, in Jurkat cells.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a key mediator of apoptosis.
  • TRAIL belongs to the TNF superfamily and plays a role in immune regulation and cell death.
  • Understanding TRAIL's mechanism in cancer cells is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the therapeutic potential of TRAIL.
  • To analyze TRAIL-induced apoptosis in Jurkat cells.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) to detect TRAIL receptors (DR4 and DR5).
  • Colony formation assay and cell counting kit to assess cytotoxic effects.
  • DNA fragmentation (DNA ladder), PI staining, and JC-1 fluorescence to evaluate apoptosis and mitochondrial membrane potential.

Main Results:

  • TRAIL significantly inhibited proliferation of Jurkat cells.
  • TRAIL induced internucleosomal DNA fragmentation, a hallmark of apoptosis.
  • TRAIL treatment led to a loss of mitochondrial membrane potential.

Conclusions:

  • Recombinant soluble TRAIL demonstrates therapeutic potential for cancer treatment.
  • TRAIL effectively induces apoptosis in cancer cells, suggesting its viability as a therapeutic agent.

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