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FGF-2 enhances vascularization for adipose tissue engineering.

Kacey G Marra¹, Alicia J DeFail, Julio A Clavijo-Alvarez

¹Pittsburgh, Pa. From the Division of Plastic Surgery, Department of Surgery and the Department of Bioengineering, University of Pittsburgh, and the McGowan Institute for Regenerative Medicine.

Plastic and Reconstructive Surgery

|March 20, 2008

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Small intestinal submucosa particles serve as a scaffold for preadipocytes, enhancing fat tissue regeneration. Fibroblast growth factor-2 delivery improved cell survival and vascularization in vivo.

Area of Science:

Biomaterials science
Tissue engineering
Regenerative medicine

Background:

Current soft-tissue reconstruction methods like flaps and implants have limitations.
Autologous fat grafting faces challenges with variable resorption.
Preadipocytes offer potential for improved fat tissue generation and survival.

Purpose of the Study:

To evaluate small intestinal submucosa (SIS) particles as injectable scaffolds for preadipocytes.
To investigate the role of fibroblast growth factor (FGF)-2 in enhancing vascularization and cell survival.

Main Methods:

Preadipocytes were isolated and cultured on SIS microparticles in a spinner flask bioreactor.
FGF-2 was encapsulated in poly(lactic-co-glycolic acid) microspheres.
The preadipocyte/SIS construct with FGF-2 microspheres was implanted in a mouse subcutaneous model.

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Main Results:

Preadipocytes successfully attached and proliferated on SIS particles in vitro, maintaining viability.
In vivo studies showed significantly enhanced vascularization with FGF-2 delivery.
Cell survival was confirmed over a 14-day observation period using fluorescent dye labeling.

Conclusions:

SIS particles provide a suitable scaffold for ex vivo preadipocyte expansion.
Injectable SIS particles support cell delivery for soft-tissue reconstruction.
FGF-2 delivery via PLGA microspheres promotes cell survival and vascularization, improving fat graft outcomes.