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Updated: Aug 10, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
CD3-induced T-cell proliferation and interleukin-2 secretion is modulated by the CD45 antigen
T Oravecz1, E Monostori, E Kurucz
1Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged.
Abstract:
In the present study we have investigated the effect of CD45, CD45RA and CD45RO monoclonal antibodies (MoAbs) on the CD3 receptor-mediated proliferation of human T lymphocytes. It is shown that CD3-induced proliferation of purified resting T cells and quiescent T lymphoblasts (QTL) is promoted via all of the investigated CD45-associated epitopes. It is also shown that the CD45 molecules are required to be cross-linked for costimulation. The MoAbs enhance the interleukin-2 (IL-2) production of CD3-stimulated QTL. The elevation of the IL-2 production correlates with the increase in CD3-induced cell proliferation suggesting that the CD45-driven regulation of T lymphocyte activation is linked to the IL-2 pathway.
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