Related Experiment Video
Updated: Jul 6, 2026

Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions
Published on: April 17, 2026
X-ray Raman spectroscopic study of water in the condensed phases
John S Tse1, Dawn M Shaw, Dennis D Klug
1Physics and Engineering Physics, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E2, Canada.
Abstract:
Oxygen K-edge x-ray absorption spectra of high-density amorphous (HDA) ice, low-density amorphous ice Ic, ice Ih, normal and deuterated liquid water were measured with the synchrotron x-ray Raman scattering method under almost identical experimental conditions by in situ heating of an HDA ice sample. The distinct preedge structure previously reported in water was observed in all the spectra. The results show that core-hole excitations are localized and not strongly affected by the local environment. Therefore, the existence of the preedge feature is not a concise indicator of the magnitude of local disorder within the hydrogen bonded network. The intensity of the near-edge absorption shifts into the postedge region when the hydrogen bond network becomes more ordered. This observation is interpreted as an enhancement of Wannier over Frenkel excitations in an ordered crystal.
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration
According to Hooke's law, the vibrational frequency is directly proportional to the...
Determination of Crystal Structures
X-ray Diffraction of Biological Samples
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
UV–Vis Spectroscopy: Molecular Electronic Transitions

