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Pathogenicity of CL-1 chicken anemia agent
C M Lamichhane1, D B Snyder, M A Goodwin
1Virginia/Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
Avian Diseases
|July 1, 1991
Summary
A novel chick anemia agent (CAA) virus was isolated from broiler chickens. This virus caused severe anemia, hemorrhage, and thymus atrophy in specific-pathogen-free chicken embryos and chicks.
Area of Science:
- Virology
- Avian Pathology
Background:
- Chick anemia virus (CAA) is an economically significant pathogen in poultry.
- Previous isolates of CAA have been identified in Europe and Japan.
Purpose of the Study:
- To isolate and characterize a novel strain of chick anemia agent (CAA).
- To investigate the pathogenicity of the isolated CL-1 CAA strain in chicken embryos and chicks.
Main Methods:
- Isolation of the virus through blind subcultures in an MDCC-MSB1 cell line.
- Inoculation of specific-pathogen-free chicken embryos via yolk-sac injection.
- Pathological examination of infected embryos and hatched chicks.
Main Results:
- A virus, designated CL-1 CAA, was isolated from broiler chickens with hemorrhagic necrotic wing tips.
- Infection of chicken embryos resulted in high mortality due to systemic hemorrhage (49%).
- Hatched chicks exhibited dwarfism, anemia, pale tissues, marked atrophy of hematopoietic organs, and severe thymus hypoplasia.
Conclusions:
- The CL-1 CAA strain is pathogenic and causes severe anemia and lymphoid atrophy in chickens.
- The isolated virus shares physicochemical and pathological characteristics with previously identified CAA isolates.
- This study contributes to understanding CAA diversity and pathogenicity.