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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

Published on: May 28, 2015

Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei

Changqing Zhang1, Roger A Barthelson, Georgina M Lambert

  • 1Department of Plant Sciences , The University of Arizona, Tucson, AZ 85721, USA.

Plant Physiology
|March 21, 2008
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...