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Related Concept Videos

Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...

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Related Experiment Video

Updated: Jul 6, 2026

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
08:21

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Published on: October 2, 2018

Multivectorial abnormal cell migration in the NOD mouse thymus.

Daniella A Mendes-da-Cruz1, Salete Smaniotto, Alexandre C Keller

  • 1Laboratory of Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. mendes-da-cruz@necker.fr

Journal of Immunology (Baltimore, Md. : 1950)
|March 21, 2008
PubMed
Summary
This summary is machine-generated.

NOD thymocyte migration is impaired due to altered expression of cell adhesion molecules and chemokine receptors. These defects in thymocyte trafficking may contribute to the development of autoimmunity in NOD mice.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Previous studies indicated fibronectin/VLA-5-dependent impairment of NOD thymocyte migration.
  • NOD thymocyte arrest in thymic perivascular spaces suggests additional migration defects.

Purpose of the Study:

  • To investigate the role of extracellular matrix ligands and CXCL12 in NOD thymocyte migration.
  • To analyze the expression of receptors and intrathymic ligand deposition.

Main Methods:

  • Immunohistochemistry to assess intrathymic CXCL12, fibronectin, and laminin.
  • Flow cytometry to determine receptor expression (VLA-5, CXCR4, VLA-6).
  • Transwell and transendothelial migration assays to measure thymocyte mobility.

Main Results:

  • NOD thymocytes exhibit significantly lower VLA-5 expression, especially CD4(+)Foxp3(+) cells.
  • Increased deposition and colocalization of CXCL12, laminin, and fibronectin in the NOD thymus.
  • Altered CXCR4 and VLA-6 expression and enhanced migration of NOD thymocytes towards CXCL12 and laminin.
  • Diminished transendothelial migration of NOD thymocytes with exogenous fibronectin.

Conclusions:

  • NOD thymocytes display multiple cell migration abnormalities, including altered expression of adhesion molecules and chemokine receptors.
  • These multifaceted migration defects may influence the onset of autoimmunity in NOD mice.