Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectral recovery by analytic continuation in crossing-based spectrum analysis.

Applied optics·2010
Same author

Single-Gaussian-beam interaction with a dielectric microsphere: radiation forces, multiple internal reflections, and caustic structures.

Applied optics·2010
Same author

Detection accuracy in zero-crossing-based spectrum analysis and image reconstruction.

Applied optics·2010
Same author

Two-dimensional image reconstruction from Fourier coefficients computed directly from zero crossings.

Applied optics·2010
Same author

Fourier transform refractometry using multichannel detection.

Applied optics·2010
Same author

Imaging properties of axicon in a scanning optical system.

Applied optics·2010

Related Experiment Video

Updated: Jul 6, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Third-harmonic generation microscopy in highly scattering media.

C M Blanca1, C Saloma

  • 1National Institute of Physics, University of the Philippines, Diliman, Quezon City 1101, Philippines.

Applied Optics
|March 21, 2008
PubMed
Summary
This summary is machine-generated.

Monte Carlo simulations reveal how third-harmonic generation (THG) microscopy performs in scattering media. This analysis details the point-spread function and temporal broadening effects, aiding in imaging applications.

More Related Videos

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

  • Biomedical Optics
  • Microscopy Techniques
  • Light Scattering

Background:

  • Third-harmonic generation (THG) microscopy offers label-free imaging capabilities.
  • Performance in scattering media remains a challenge for THG microscopy.
  • Understanding light propagation is crucial for optimizing imaging in biological tissues.

Purpose of the Study:

  • To analyze the performance of third-harmonic generation (THG) microscopy in highly scattering media.
  • To derive the three-dimensional point-spread function (PSF) of THG microscopy under various scattering conditions.
  • To investigate the impact of temporal broadening on the THG signal.

Main Methods:

  • Utilized the Monte Carlo technique to simulate light propagation in scattering media.
  • Derived the PSF for laser-scanning THG microscopy with pulsed excitation.
  • Analyzed scattering depth (h) relative to the mean free path (d(s)) for isotropic and anisotropic media.
  • Considered signal detection using a large-area photodetector.

Main Results:

  • The PSF of THG microscopy was determined by the third power of the normalized excitation beam distribution.
  • Temporal broadening of the excitation pulse was analyzed as a function of h/d(s).
  • Performance limitations and characteristics in scattering environments were quantified.

Conclusions:

  • The study provides a theoretical framework for understanding THG microscopy in scattering media.
  • Results aid in optimizing THG microscopy for biological imaging applications.
  • Comparison with two-photon fluorescence microscopy highlights relative strengths and weaknesses.