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Related Experiment Video

Updated: Jul 6, 2026

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
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Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Published on: June 25, 2015

Gene regulation technologies in zebrafish.

Hanife Esengil1, James K Chen

  • 1Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Molecular Biosystems
|March 21, 2008
PubMed
Summary
This summary is machine-generated.

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Developing reverse-genetic tools is crucial for unlocking the full potential of zebrafish models in biomedical research. New technologies, like conditional transgene expression systems, will advance studies of human physiology and disease.

Area of Science:

  • Genetics
  • Developmental Biology
  • Biomedical Research

Background:

  • Zebrafish are valuable models in biomedical research.
  • The full potential of zebrafish models is limited by a lack of reverse-genetic resources.
  • Reverse genetics allows for targeted gene manipulation to study function.

Purpose of the Study:

  • To review current technologies for genetic perturbations in zebrafish.
  • To highlight the importance of reverse-genetic resources for zebrafish model development.
  • To discuss the potential of these technologies for studying human physiology and disease.

Main Methods:

  • Review of existing literature on genetic perturbation technologies in zebrafish.
  • Description of an ecdysone receptor-based system for conditional transgene expression.

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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
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Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

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Last Updated: Jul 6, 2026

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
10:22

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Published on: June 25, 2015

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

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09:53

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Published on: June 3, 2017

  • Discussion of the application of these methods in creating novel zebrafish models.

    • Main Results:

    • Several technologies for genetic perturbations in zebrafish are available.
    • An ecdysone receptor-based system enables conditional transgene expression.
    • These tools facilitate the development of advanced zebrafish models.

    Conclusions:

    • The development of reverse-genetic resources is essential for maximizing the utility of zebrafish in research.
    • Conditional transgene expression systems offer powerful new avenues for genetic studies in zebrafish.
    • These advancements will accelerate the creation of zebrafish models for investigating human health and disease.