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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Updated: Jul 6, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

DNA separation methodology based on charge neutralization in a polycationic gel matrix.

Gleb Zilberstein1, Leonid Korol, Joanna Znaleziona

  • 1Cleardirection Ltd., 4 Pekeris Street, Rehovot 76702, Israel.

Analytical Chemistry
|March 22, 2008
PubMed
Summary
This summary is machine-generated.

A new DNA separation technique uses charged polyacrylamide gels to resolve fragments with high precision. This method significantly improves upon existing techniques for genetic analysis and forensic applications.

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Electrophoretic Separation of Proteins
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Electrophoretic Separation of Proteins

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Last Updated: Jul 6, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
12:03

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Published on: February 24, 2011

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Conventional polyacrylamide gel electrophoresis (PAGE) has limitations in resolving small DNA fragments.
  • Existing DNA separation methods struggle with high-resolution discrimination of closely sized fragments.

Purpose of the Study:

  • To introduce a novel method for DNA fragment separation using polycationic polyacrylamide gels.
  • To demonstrate the enhanced resolution capabilities of this new technique for various DNA fragment lengths.

Main Methods:

  • Incorporation of positively charged monomers (Immobilines) into a neutral polyacrylamide backbone to create a polycationic gel.
  • Two separation modes: migration against a positive charge gradient for focusing, or in a plateau gel with constant charge.
  • Utilizing differential charge modulation for separation in plateau gels.

Main Results:

  • Achieved separation of DNA fragments differing in length by <0.5% in the 100-1000 bp range.
  • Resolved a 656- and 659-bp DNA fragment doublet, unresolvable by conventional PAGE.
  • Successfully resolved single nucleotide polymorphisms (SNPs) in the 10-100 bp range using steeper charge gradients.

Conclusions:

  • The novel polycationic gel electrophoresis method offers superior resolution for DNA fragment separation.
  • This technique provides a significant advancement over existing methods for molecular biology, forensic medicine, and genetic screening.