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Related Experiment Video

Updated: Jul 6, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Exon-level expression profiling: a comprehensive transcriptome analysis of oral fluids.

Zhanzhi Hu1, Bernhard G Zimmermann, Hui Zhou

  • 1Dental Research Institute, 73-017 Center for Health Sciences, University of California, Los Angeles, CA 90095-1668, USA.

Clinical Chemistry
|March 22, 2008
PubMed
Summary

This study developed a method to analyze fragmented RNA in saliva for gene expression profiling. This approach enables high-resolution transcriptome analysis and validation of multiple targets from small samples.

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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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Published on: January 22, 2014

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Last Updated: Jul 6, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Area of Science:

  • Molecular Biology
  • Genomics
  • Biomarker Discovery

Background:

  • Saliva gene expression profiling is limited by fragmented RNA and small sample volumes.
  • Existing technologies struggle with amplification and detection of degraded salivary RNAs.
  • Quantitative PCR (qPCR) validation is challenging with limited saliva sample sizes.

Purpose of the Study:

  • To demonstrate the feasibility of combining universal mRNA amplification with exon arrays for candidate selection.
  • To validate a multiplex preamplification method for efficient target validation.
  • To establish a robust method for salivary transcriptome analysis despite RNA degradation.

Main Methods:

  • Utilized a universal mRNA-specific linear-amplification strategy with Affymetrix Exon Arrays.
  • Amplified nanogram-scale salivary RNA from 18 healthy individuals.
  • Employed multiplex preamplification and enzymatic cleanup for qPCR validation.

Main Results:

  • Defined a salivary exon core transcriptome (SECT) of 851 highly correlated transcripts.
  • Verified a subset of SECT transcripts via qPCR.
  • Identified and validated sex-specific salivary exon biomarkers.

Conclusions:

  • High-resolution transcriptome profiling is achievable with fragmented RNA samples.
  • Multiple targets can be validated from limited sample amounts using the developed method.
  • This approach overcomes key limitations in salivary RNA analysis.