Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-field five-dimensional fluorescence microscopy combining lifetime and spectral resolution with optical sectioning.

Optics letters·2007
Same author

Fluorescence lifetime system for microscopy and multiwell plate imaging with a blue picosecond diode laser.

Optics letters·2007
Same author

Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells.

Applied optics·2007
Same author

Enhanced fluorescence cell imaging with metal-coated slides.

Biophysical journal·2006
Same author

Diode-pumped passively mode-locked Nd:YVO4 laser at 914 nm.

Optics letters·2006
Same author

Fluorescence lifetime imaging with a low-repetition-rate passively mode-locked diode-pumped Nd:YVO4 oscillator.

Optics letters·2005

Related Experiment Video

Updated: Jul 6, 2026

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time
09:56

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

Published on: November 4, 2014

Three-dimensional acousto-optic imaging in biological tissues with parallel signal processing.

S Lévêque-Fort

    Applied Optics
    |March 22, 2008
    PubMed
    Summary

    A novel acousto-optic method reveals optical contrasts in thick biological tissues using laser light and ultrasound. This technique achieves millimeter resolution, enabling imaging of absorbing objects within 35-mm-thick samples.

    More Related Videos

    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
    06:40

    Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

    Published on: June 8, 2022

    Related Experiment Videos

    Last Updated: Jul 6, 2026

    Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time
    09:56

    Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time

    Published on: November 4, 2014

    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
    06:40

    Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

    Published on: June 8, 2022

    Area of Science:

    • Biomedical Optics
    • Acousto-Optics
    • Medical Imaging

    Background:

    • Optical imaging in thick biological tissues is challenging due to light scattering.
    • Existing methods often lack sufficient resolution or penetration depth.

    Purpose of the Study:

    • To develop and demonstrate a novel acousto-optic method for imaging within thick biological tissues.
    • To achieve millimeter-sized resolution and significant optical contrast enhancement.

    Main Methods:

    • Utilized an acousto-optic technique based on the interaction of scattered laser light with a focused ultrasonic field.
    • Employed a parallel-processing approach for speckle demodulation to enhance modulation depth.
    • Investigated the relationship between speckle modulation depth and local optical properties.

    Main Results:

    • Successfully revealed optical contrasts in biological tissues several centimeters thick.
    • Achieved millimeter-sized resolution in imaging.
    • Demonstrated the ability to image optically absorbing objects within 35-mm-thick biological tissues.
    • Improved the observed degree of modulation by 2 orders of magnitude.

    Conclusions:

    • The described acousto-optic method offers a promising approach for high-resolution imaging in deep biological tissues.
    • The parallel-processing demodulation significantly enhances image quality and statistical reliability.
    • This technique has potential applications in biomedical diagnostics and research.