Depolarization of autofluorescence from malignant and normal human breast tissues

We report on steady-state measurements on the anisotropy of autofluorescence from malignant and normal breast tissue as a function of tissue thickness. For thin tissue sections the anisotropy from normal tissue was found to be smaller compared with that from malignant tissue. However, the opposite result was obtained for thicker tissues. A phenomenological model was also developed to simulate the dependence of anisotropy on tissue thickness.