Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Local optical parameters of spherical polydispersions: simple approximations.

Applied optics·2010

Same author

Analytical solution to the optical transfer function of a scattering medium with large particles.

Applied optics·2010

Same author

Angular spectrum of light transmitted through turbid media: theory and experiment.

Applied optics·2008

Same author

Physically based parameterizations of the short-wave radiative characteristics of weakly absorbing optically thick media: application to liquid-water clouds.

Applied optics·2008

Same author

Integral light-scattering and absorption characteristics of large, nonspherical particles.

Applied optics·2008

Same author

Statistical properties of light reflected and transmitted by a thick horizontally inhomogeneous turbid layer.

Journal of the Optical Society of America. A, Optics, image science, and vision·2005

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Multiple light scattering in laser particle sizing.

A A Kokhanovsky¹, R Weichert

¹Institute of Particle Technology and Environmental Engineering, Technical University Clausthal, Leibnizstrasse 19, D-38678 Clausthal-Zellerfeld, Germany. alex@zege.bas-net.by

|March 22, 2008

Summary

This summary is machine-generated.

Accurate particle size measurement using laser diffraction spectrometry requires accounting for multiple light scattering. Ignoring this phenomenon leads to underestimation, but advanced algorithms improve retrieval accuracy.

More Related Videos

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Related Experiment Videos

Last Updated: Jul 6, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Area of Science:

Physics
Optical Science
Materials Science

Background:

Multiple light scattering significantly impacts laser diffraction spectrometry.
Current laser particle sizers often neglect multiple scattering effects.
This omission leads to underestimation of particle sizes at high concentrations.

Purpose of the Study:

To evaluate the influence of multiple light scattering on transmitted light through scattering layers.
To analyze the relationships between different theories for multiple scattering in laser particle sizing.

Main Methods:

Investigated the effects of multiple light scattering on laser diffraction measurements.
Analyzed theoretical frameworks for incorporating multiple scattering corrections.
Evaluated the impact on particle size retrieval accuracy.

Main Results:

Multiple light scattering causes underestimation of particle sizes in disperse media.
Processing data with multiple-scattering algorithms improves retrieval accuracy.
Established relationships among various theoretical approaches to multiple scattering.

Conclusions:

Accounting for multiple light scattering is crucial for accurate laser particle sizing.
Advanced algorithms are necessary to correct for these effects.
Understanding theoretical relationships enhances the reliability of particle size determination.