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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Phase-Contrast Microscopes
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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Easy and Accurate Mechano-profiling on Micropost Arrays
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Published on: November 17, 2015

Color-coded optical profilometry with >10(6) resolved depth steps.

E Hasman, V Kleiner

    Applied Optics
    |March 22, 2008
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new method for fast, real-time 3D surface measurement using color-coded light-stripe triangulation. The technique achieves high resolution over a large depth range, enabling detailed surface topography analysis.

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    Published on: September 26, 2019

    Area of Science:

    • Optics and Photonics
    • Metrology
    • Surface Science

    Background:

    • Accurate 3D surface topography is crucial for various scientific and industrial applications.
    • Traditional triangulation methods often face limitations in depth range, resolution, or speed.
    • Developing advanced optical metrology techniques is essential for high-precision surface characterization.

    Purpose of the Study:

    • To present a novel light-stripe triangulation configuration for enhanced 3D surface topography measurement.
    • To analyze and experimentally demonstrate a new approach for parallel, fast, real-time 3D surface profiling.
    • To achieve a large number of optically resolved depth steps without compromising resolution.

    Main Methods:

    • Utilized a color-coding and decoding arrangement for light-stripe triangulation.
    • Employed polychromatic illumination and axially dispersing optical elements.
    • Developed a parallel processing approach for real-time data acquisition.

    Main Results:

    • Achieved real-time 3D surface topography measurements.
    • Demonstrated a significant increase in depth-measuring range without loss of axial or lateral resolution.
    • Obtained 3D surface measurements with lateral and depth optical resolutions of <40 nm over a 48 mm depth of focus.
    • Generated 1.2 x 10^6 resolving depth steps.

    Conclusions:

    • The novel light-stripe triangulation method offers a powerful tool for high-resolution 3D surface metrology.
    • The technique enables efficient and accurate surface topography mapping over extended depth ranges.
    • This advancement has potential applications in fields requiring precise surface analysis.