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10:28
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization
Rosemeire de Lordo Franco1, Roberto Chiesa, Marcio Mateus Beloti
1Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Brazil.
Journal of Biomedical Materials Research. Part A
|March 22, 2008
Summary
The anodized alkali-treated titanium (BSP-AK) surface enhanced human osteoblastic cell proliferation and differentiation. This suggests BSP-AK titanium is promising for dental and orthopedic implants.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Titanium (Ti) is widely used for dental and orthopedic implants due to its biocompatibility.
- Surface modification of titanium is crucial for improving osseointegration and implant success.
- The anodized alkali treatment (BSP-AK) is a novel surface modification technique.
Purpose of the Study:
- To evaluate the in vitro osteogenic potential of human osteoblastic cells on a BSP-AK modified titanium surface.
- To compare the BSP-AK titanium surface with a machined titanium surface (control).
- To assess the suitability of BSP-AK titanium for dental and orthopedic applications.
Main Methods:
- Subculturing human osteoblastic cells derived from alveolar bone.
- Seeding cells on BSP-AK and machined Ti discs.
- Culturing cells under osteogenic conditions for up to 21 days.
- Employing morphologic and biochemical assays to evaluate cell behavior and matrix formation.
Main Results:
- No significant differences in cell attachment or mineralized matrix formation were observed between BSP-AK and control Ti surfaces.
- A significant increase in cell population, alkaline phosphatase (ALP) activity, and collagen content was detected on the BSP-AK surface.
- Human osteoblastic cells showed sensitivity to the BSP-AK surface during the proliferative to differentiation transition.
Conclusions:
- The BSP-AK modified titanium surface promotes osteoblastic cell proliferation and differentiation in vitro.
- The enhanced cellular response suggests improved osseointegration potential.
- BSP-AK treatment, combined with its mechanical properties, shows promise for developing advanced dental and orthopedic implant surfaces.
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