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Thermal biosensor for detecting nonylphenol in the environment.

A V Demchenko1, V G Melnik, N F Starodub

  • 1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. demchencoa@ukr.net

Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
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A new thermal biosensor was developed for detecting nonylphenol (NPh). This method offers a simpler, field-applicable alternative to SPR biosensors, despite lower sensitivity.

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Nonylphenol (NPh) is an environmental pollutant requiring sensitive detection methods.
  • Surface Plasmon Resonance (SPR) biosensors offer high sensitivity but can be complex for field use.
  • Previous SPR-based immune biosensors achieved NPh detection at ng/ml levels within 10 minutes.

Purpose of the Study:

  • To develop a thermal immune biosensor for sensitive and specific nonylphenol (NPh) determination.
  • To create a biosensor suitable for field applications, prioritizing robustness and ease of use.

Main Methods:

  • Development of a thermal biosensor measuring heat released from hapten-antibody interactions.
  • Utilized direct detection of NPh.
  • Compared performance with existing SPR-based immune biosensors.

Main Results:

  • The thermal biosensor achieved direct NPh detection with a sensitivity of approximately 1 microg/ml.
  • The total analysis time for the thermal biosensor was 20-30 minutes.
  • The thermal biosensor demonstrated reduced sensitivity to sample admixtures and simpler operation compared to SPR.

Conclusions:

  • The developed thermal biosensor is a viable tool for NPh detection, particularly for field conditions.
  • Its robustness and ease of use make it a practical alternative to SPR biosensors for environmental monitoring.
  • Further optimization may enhance sensitivity for broader applications.