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Updated: Jul 6, 2026
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
[Expression of peroxisome proliferator-activated receptor gamma in glioma]
Ming-hua Wang1, Xue-yun Zhong, Chen-li Lin
1Department of Pathology, Chongqing Medical Oniversity, Chongqing 410016, China. wangmhhh@yahoo.cn
Objective:
To investigate the expression and significance of peroxisome proliferators-activated receptor gamma (PPAR gamma) in human glioma.
Methods:
Immunohistochemical staining for PPAR gamma was performed using biopsy specimens of human glioma of various histological types. Expression of PPAR gamma and GFAP in glioma cell lines SWO-38, U251 and SHG-44 were analyzed using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
Results:
Immunohistochemical study showed that PPAR gamma was expressed in glioma tissues with positive rate of 37.5%. Western blotting and RT-PCR showed that PPAR gamma was expressed in both glioma cell lines SWO-38 and U251, but not in SHG-44 cells. However, high expression of GFAP was detected in SHG-44 cells.
Conclusion:
PPAR gamma is associated with carcinogens of glioma. Actived PPAR gamma by agonist may be a novel approach to the treatment of glioma.
