[Matrine-induced erythroid differentiation of K562 cells is associated with activation of the apoptotic pathway]
Cui-mei Zhang1, Jian-hui Gao, De-le Li
1Department of Child Health Care, Zhongshan Bo'ai Hospital, Southern Medical University, Zhongshan 528403, China. zhangcuimei0527@163.com
Objective:
To observe matrine-induced erythroid differentiation of K562 cells in relation to activation of the apoptotic pathway in vitro.
Methods:
K562 cells were cultured in the presence or absence of matrine at different concentrations for 4 days, and the morphological and ultramicrostructural changes of the cells were observed using inverted microscopy and transmission electron microscopy, respectively. The expression of apoptosis-related protein p27kip1 was detected by immunocytochemistry.
Results:
Compared to untreated K562 cells, the cells treated with matrine at 0.10 g/L exhibited apoptostic characteristics in the cellular morphology and ultramicrostructure, with the expression of p27kip1 protein upregulated in a concentration- and time-dependent manner.
Conclusion:
Matrine-induced erythroid differentiation of K562 cells is associated with cell apoptosis, and upregulation of p27kip1 protein expression may play a crucial role in the process of apoptosis.
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