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Updated: Jul 6, 2026
11:24
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms
Mark R Liles1, Lynn L Williamson, Jitsupang Rodbumrer
1Department of Biological Sciences, Auburn University, Auburn, Alabama, USA.
Applied and Environmental Microbiology
|March 25, 2008
Abstract:
We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.
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