Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

NMR Spectrometers: Resolution and Error Correction

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Introducing a Novel Course-Based Undergraduate Research Experience Using Duckweed as a Model System.

Integrative organismal biology (Oxford, England)·2026

Same author

[Chinese Expert Consensus on GnRH-a in Obstetrics and Gynecology].

Zhonghua fu chan ke za zhi·2025

Same author

[High position dacryocystorhinostomy for the treatment of chronic dacryocystitis following failed nasolacrimal duct stent implantation].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025

Same author

[The nomogram prediction model for the risk of dropout in sublingual immunotherapy of patients with allergic rhinitis].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025

Same author

[Risk factors of coronal imbalance following posterior lumbar short-level fixation and fusion for degenerative lumbar scoliosis].

Zhonghua yi xue za zhi·2025

Same author

[Application value of domestic single-port robot in nephron sparing surgery].

Zhonghua yi xue za zhi·2024

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Superresolution Near-field Readout in Phase-Change Optical Disk Data Storage.

|March 25, 2008

Summary

Super-resolution near-field structure (Super-RENS) technology enables optical disk readout beyond the diffraction limit. Different Super-RENS designs yield varying signal strengths, with metallic-region types showing superior performance for high-density data storage.

Area of Science:

Optical data storage
Nanotechnology
Diffraction theory

Background:

Traditional optical disk readout is limited by the diffraction of light.
Superresolution (SR) near-field structures (Super-RENS) offer a potential solution to overcome these limitations.
Theoretical examination is crucial for understanding and optimizing Super-RENS performance.

Purpose of the Study:

To theoretically investigate the readout characteristics of phase-change optical disks utilizing Super-RENS.
To analyze the factors influencing the read signal and spatial resolution of Super-RENS systems.
To compare the performance of different Super-RENS configurations.

Main Methods:

Application of three-dimensional, full-wave vector diffraction theory.

More Related Videos

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Theoretical modeling of Super-RENS structures and optical disk layers.

Simulation of laser beam interaction with the disk surface and SR elements.

Main Results:

Super-RENS technology demonstrates spatial resolution exceeding the diffraction limit.
Readout signal quality depends on SR material, disk structure, and laser polarization.
Antimony-based Super-RENS yielded low signals (~30 dB CNR for 300 nm marks).
Super-RENS with metallic region formation showed significantly higher signals (~50 dB CNR for 300 nm marks).

Conclusions:

Super-RENS is a viable technology for achieving ultra-high-density optical data storage.
The design of the SR layer is critical for achieving high carrier-to-noise ratios (CNR).
Metallic-region-forming Super-RENS offers superior readout performance compared to antimony-based systems.