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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Updated: Jul 6, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Fast multi-tau real-time software correlator for dynamic light scattering.

D Magatti, F Ferri

    Applied Optics
    |March 25, 2008
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a PC-based software correlator for dynamic light scattering, enabling real-time data processing. The developed correlator accurately determines particle sizes from light scattering data with high precision.

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    Last Updated: Jul 6, 2026

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    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

    Published on: December 11, 2021

    Area of Science:

    • Physics
    • Materials Science
    • Chemistry

    Background:

    • Dynamic light scattering (DLS) is a powerful technique for measuring particle size distributions.
    • Real-time processing of DLS data can significantly improve experimental efficiency.
    • Existing correlator hardware can be expensive and complex.

    Purpose of the Study:

    • To develop a cost-effective, PC-based multi-tau software correlator for DLS data.
    • To enable on-line processing of DLS data without hard disk storage.
    • To achieve high precision in particle size determination using DLS.

    Main Methods:

    • Development of a LabVIEW-based multi-tau software algorithm for real-time data processing.
    • Utilized a standard photon-counting unit and a National Instruments timer-counter.
    • Tested with dilute solutions of calibrated polystyrene spheres.

    Main Results:

    • Achieved real-time correlation function calculation down to ~5 microseconds.
    • Enabled batch processing down to ~300 nanoseconds time scales.
    • Demonstrated high precision in particle diameter recovery (within a few percent rms).

    Conclusions:

    • The developed PC-based software correlator is suitable for efficient and precise DLS data analysis.
    • The system offers a cost-effective alternative to traditional hardware correlators.
    • The algorithm's performance is robust and adaptable to future technological advancements.