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Updated: Jul 6, 2026
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
Published on: October 31, 2012
Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human
Federica Alciato1, Pier Paolo Sainaghi, Luigi Castello
1Department of Clinical and Experimental Medicine, Università del Piemonte Orientale, Novara, Italy.
Abstract:
Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3+/-3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.
